2pi4

X-ray diffraction
2.5Å resolution

T7RNAP complexed with a phi10 protein and initiating GTPs.

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
T7 RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 878 amino acids
Theoretical weight: 98.41 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 6-883; Coverage: 99%)
Gene name: 1
Sequence domains:
Structure domains:
5'-D(*CP*TP*TP*CP*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP*AP*TP*TP*A)-3' Chain: T
Molecule details ›
Chain: T
Length: 22 nucleotides
Theoretical weight: 6.73 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
5'-D(*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*T)-3' Chain: P
Molecule details ›
Chain: P
Length: 14 nucleotides
Theoretical weight: 4.22 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P21212
Unit cell:
a: 224.45Å b: 73.791Å c: 80.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.265 0.297
Expression systems:
  • Escherichia coli
  • Not provided