› 2phy

X-ray diffraction
1.4Å resolution

PHOTOACTIVE YELLOW PROTEIN, DARK STATE (UNBLEACHED)

Released:
DOI: 10.2210/pdb2phy/pdb
PDB 2phy coloured by chain and viewed from the front.
PDB 2phy coloured by chain and viewed from the side.
PDB 2phy coloured by chain and viewed from the top.
Source organism: Halorhodospira halophila
Primary publication:
1.4 A structure of photoactive yellow protein, a cytosolic photoreceptor: unusual fold, active site, and chromophore.
Borgstahl GE, Williams DR, Getzoff ED
Biochemistry 34 6278-87 (1995)
PMID: 7756254

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P63
Unit cell:
a: 66.9Å b: 66.9Å c: 40.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.226

Quick links

Citations

34 review citations

Photoreaction Mechanisms of Flavoprotein Photoreceptors and Their Applications.
Iwata et al. (2021)
33 more

37 mentions without citation

Small Molecule-Photoactive Yellow Protein Labeling Technology in Live Cell Imaging.
Gao et al. (2016)
36 more