X-ray diffraction
1.4Å resolution

Crystal structrue of deletion mutant of APS-kinase domain of human PAPS-synthetase 1 in complex with cyclic PAPS and dADP


Function and Biology Details

Reactions catalysed:
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 179 amino acids
Theoretical weight: 19.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O43252 (Residues: 51-226; Coverage: 28%)
Gene names: ATPSK1, PAPSS, PAPSS1
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 44.09Å b: 59.4Å c: 139.02Å
α: 90° β: 90° γ: 90°
R R work R free
0.197 0.194 0.22
Expression system: Escherichia coli