2pd6

X-ray diffraction
2Å resolution

Structure of human hydroxysteroid dehydrogenase type 8, HSD17B8

Released:
Source organism: Homo sapiens
Entry authors: Turnbull AP, Salah E, Gileadi O, Savitsky P, Guo K, Bunkoczi G, Pike ACW, Ugochukwu E, Umeano C, von Delft F, Weigelt J, Arrowsmith CH, Sundstrom M, Edwards A, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed:
Testosterone + NAD(+) = androstenedione + NADH
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(3R)-3-hydroxyacyl-CoA dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 264 amino acids
Theoretical weight: 27.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92506 (Residues: 6-261; Coverage: 98%)
Gene names: FABGL, HKE6, HSD17B8, RING2, SDR30C1
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 68.839Å b: 62.429Å c: 102.439Å
α: 90° β: 99.22° γ: 90°
R-values:
R R work R free
0.186 0.184 0.227
Expression system: Escherichia coli