› 2pbo

X-ray diffraction
1.85Å resolution

E27Q mutant of EXO-B-(1,3)-Glucanase from Candida Albicans at 1.85 A

Released:
DOI: 10.2210/pdb2pbo/pdb
PDB entry 2pbo coloured by chain, front view.
PDB entry 2pbo coloured by chain, side view.
PDB entry 2pbo coloured by chain, top view.
Source organism: Candida albicans
Entry authors: Cutfield SM, Cutfield JF, Patrick WM

Function and Biology Details

Reaction catalysed: 
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151564 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucan 1,3-beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 45.79 KDa
Source organism: Candida albicans
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29717 (Residues: 39-438; Coverage: 96%)
Gene names: CAALFM_C102990CA, Ca49C10.05, CaO19.10507, CaO19.2990, EXG, EXG1, XOG, XOG1
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 60Å b: 65.17Å c: 96.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.13 0.158
Expression system: Saccharomyces cerevisiae

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