2pbd

X-ray diffraction
1.5Å resolution

Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Profilin-1 Chain: P
Molecule details ›
Chain: P
Length: 139 amino acids
Theoretical weight: 14.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07737 (Residues: 2-140; Coverage: 99%)
Gene name: PFN1
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic
Vasodilator-stimulated phosphoprotein Chain: V
Molecule details ›
Chain: V
Length: 43 amino acids
Theoretical weight: 3.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P50552 (Residues: 203-245; Coverage: 11%)
Gene name: VASP

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 119.094Å b: 56.589Å c: 75.227Å
α: 90° β: 104.75° γ: 90°
R-values:
R R work R free
0.158 0.156 0.19
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided