› 2p9h

X-ray diffraction
2Å resolution

High resolution structure of the Lactose Repressor bound to IPTG

Released:
DOI: 10.2210/pdb2p9h/pdb
PDB 2p9h coloured by chain and viewed from the front.
PDB 2p9h coloured by chain and viewed from the side.
PDB 2p9h coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structural analysis of lac repressor bound to allosteric effectors.
Daber R, Stayrook S, Rosenberg A, Lewis M
J. Mol. Biol. 370 609-19 (2007)
PMID: 17543986

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 28.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03023 (Residues: 62-330; Coverage: 75%)
Gene names: JW0336, b0345, lacI
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand IPT 2 x IPT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P41212
Unit cell:
a: 77.125Å b: 77.125Å c: 210.583Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Transcription control engineering and applications in synthetic biology.
Engstrom et al. (2017)
4 more

7 mentions without citation

Engineering an allosteric transcription factor to respond to new ligands.
Taylor et al. (2016)
6 more