X-ray diffraction
2Å resolution

High resolution structure of the Lactose Repressor bound to IPTG

Source organism: Escherichia coli
Primary publication:
Structural analysis of lac repressor bound to allosteric effectors.
J. Mol. Biol. 370 609-19 (2007)
PMID: 17543986

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Lactose operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 28.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P03023 (Residues: 62-330; Coverage: 75%)
Gene names: JW0336, b0345, lacI
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P41212
Unit cell:
a: 77.125Å b: 77.125Å c: 210.583Å
α: 90° β: 90° γ: 90°
R R work R free
0.188 0.185 0.246
Expression system: Escherichia coli BL21(DE3)