2p8r

X-ray diffraction
2.1Å resolution

Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant

Released:
Source organism: Caenorhabditis elegans
Entry authors: Zhang L, Shen J, Guarnieri MT, Heroux A, Yang K, Zhao R

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-splicing factor 8 homolog Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 31.17 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P34369 (Residues: 2057-2329; Coverage: 12%)
Gene names: C50C3.6, prp-8
Sequence domains:
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 65.225Å b: 65.225Å c: 136.437Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.248
Expression system: Escherichia coli