Structure analysis

Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4

X-ray diffraction
2.6Å resolution
Source organism: Escherichia coli
Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 12600 Å2
Buried surface area: 1600 Å2
Dissociation area: 750 Å2
Dissociation energy (ΔGdiss): -1 kcal/mol
Dissociation entropy (TΔSdiss): 11 kcal/mol
Interface energy (ΔGint): -12 kcal/mol
Symmetry number: 1
Assembly 2
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Multimeric state: hetero tetramer

Binding statistics and energies are not available for this assembly

Macromolecules

Chain: A
Length: 158 amino acids
Theoretical weight: 18.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFX4 (Residues: 1-158; Coverage: 100%)
Gene names: JW3959, b3995, rsd
Pfam: Regulator of RNA polymerase sigma(70) subunit, Rsd/AlgQ
InterPro:
CATH: Regulator of RNA polymerase sigma(70) subunit, domain 4

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Chain: B
Length: 68 amino acids
Theoretical weight: 7.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0P6L9 (Residues: 546-613; Coverage: 11%)
Gene name: rpoD
Pfam: Sigma-70, region 4
InterPro:
CATH: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
SCOP: Sigma4 domain

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