2p7v

X-ray diffraction
2.6Å resolution

Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulator of sigma D Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 18.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFX4 (Residues: 1-158; Coverage: 100%)
Gene names: JW3959, b3995, rsd
Sequence domains: Regulator of RNA polymerase sigma(70) subunit, Rsd/AlgQ
Structure domains: Regulator of RNA polymerase sigma(70) subunit, domain 4
RNA polymerase sigma factor RpoD Chain: B
Molecule details ›
Chain: B
Length: 68 amino acids
Theoretical weight: 7.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0P6L9 (Residues: 546-613; Coverage: 11%)
Gene name: rpoD
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, APS BEAMLINE 31-ID
Spacegroup: P64
Unit cell:
a: 84.111Å b: 84.111Å c: 84.219Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.239 0.273
Expression system: Escherichia coli