› 2p7t

X-ray diffraction
2.05Å resolution

Crystal Structure of KcsA mutant

Released:
DOI: 10.2210/pdb2p7t/pdb
PDB 2p7t coloured by chain and viewed from the front.
PDB 2p7t coloured by chain and viewed from the side.
PDB 2p7t coloured by chain and viewed from the top.
Source organisms:
Entry authors: Cordero-Morales JF, Vishwanath J, Lewis A, Valeria VR, Cortes DM, Roux B, Perozo E

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
hetero dimer
hetero dodecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB-A Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
FAB-B Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 103 amino acids
Theoretical weight: 10.94 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 22-124; Coverage: 64%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand F09 1 x F09
Ligand K 6 x K
Ligand 1EM 1 x 1EM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I4
Unit cell:
a: 156.339Å b: 156.339Å c: 76.001Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.234
Expression system: Escherichia coli

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