› 2p7s

X-ray diffraction
1.8Å resolution

Enzymatic and Structural Characterisation of Amphinase, a Novel Cytotoxic Ribonuclease from Rana pipiens Oocytes

Released:
DOI: 10.2210/pdb2p7s/pdb
PDB entry 2p7s coloured by chain, front view.
PDB entry 2p7s coloured by chain, side view.
PDB entry 2p7s coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
Enzymatic and structural characterisation of amphinase, a novel cytotoxic ribonuclease from Rana pipiens oocytes.
Singh UP, Ardelt W, Saxena SK, Holloway DE, Vidunas E, Lee HS, Saxena A, Shogen K, Acharya KR
J Mol Biol 371 93-111 (2007)
PMID: 17560606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160935 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Amphinase-2 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 13.11 KDa
Source organism: Lithobates pipiens
UniProt:
  • Canonical: P85073 (Residues: 1-114; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand ACT 2 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P41
Unit cell:
a: 53.967Å b: 53.967Å c: 47.796Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.221 0.241

Quick links

Citations

10 review citations

Ribonucleases as potential modalities in anticancer therapy.
Ardelt et al. (2009)
9 more

3 mentions without citation

Evasion of ribonuclease inhibitor as a determinant of ribonuclease cytotoxicity.
Rutkoski et al. (2008)
2 more