2p6z

X-ray diffraction
1.93Å resolution

Enzymatic and Structural Characterisation of Amphinase, a Novel Cytotoxic Ribonuclease from Rana pipiens Oocytes

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amphinase-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.24 KDa
Source organism: Rana pipiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P85073 (Residues: 1-114; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P1
Unit cell:
a: 29.795Å b: 44.607Å c: 46.054Å
α: 116.56° β: 83.19° γ: 103.68°
R-values:
R R work R free
0.148 0.146 0.191
Expression system: Escherichia coli