PDBe 2p6r

X-ray diffraction
3Å resolution

Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA

Released:
Source organism: Archaeoglobus fulgidus
Primary publication:
Structural basis for DNA duplex separation by a superfamily-2 helicase.
Nat. Struct. Mol. Biol. 14 647-52 (2007)
PMID: 17558417

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
afUHEL308 HELICASE Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 79.12 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
Structure domains:
25-MER Chain: X
Molecule details ›
Chain: X
Length: 25 nucleotides
Theoretical weight: 7.66 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-D(*CP*TP*AP*GP*AP*GP*AP*CP*TP*AP*TP*CP*GP*AP*T)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6122
Unit cell:
a: 138.379Å b: 138.379Å c: 252.973Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.275
Expression systems:
  • Escherichia coli
  • Not provided