PDBe 2p5u

X-ray diffraction
2.37Å resolution

Crystal structure of Thermus thermophilus HB8 UDP-glucose 4-epimerase complex with NAD

Released:
Source organism: Thermus thermophilus HB8
Entry authors: Fu Z-Q, Chen L, Ebihara A, Shinkai A, Kuramitsu S, Yokoyama S, Zhao M, Dillard B, Chrzas J, Rose JP, Wang B-C, Southeast Collaboratory for Structural Genomics (SECSG), RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed:
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epimerase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 311 amino acids
Theoretical weight: 33.82 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKQ2 (Residues: 1-311; Coverage: 100%)
Gene name: TTHA0591
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 250.922Å b: 250.922Å c: 121.036Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.235 0.261
Expression system: Escherichia coli