› 2p5n

X-ray diffraction
1.8Å resolution

Crystal structure of mouse 17-alpha hydroxysteroid dehydrogenase in complex with coenzyme NADPH

Released:
DOI: 10.2210/pdb2p5n/pdb
PDB 2p5n coloured by chain and viewed from the front.
PDB 2p5n coloured by chain and viewed from the side.
PDB 2p5n coloured by chain and viewed from the top.
Source organism: Mus musculus
Primary publication:
Structure of 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21) holoenzyme from an orthorhombic crystal form: an insight into the bifunctionality of the enzyme.
Dhagat U, Carbone V, Chung RP, Schulze-Briese C, Endo S, Hara A, El-Kabbani O
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63 825-30 (2007)
PMID: 17909281

Function and Biology Details

Reaction catalysed: 
Androsterone + NAD(P)(+) = 5-alpha-androstane-3,17-dione + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldo-keto reductase family 1 member C21 Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.92 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91WR5 (Residues: 1-323; Coverage: 100%)
Gene name: Akr1c21
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
1 bound ligand:
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 84.91Å b: 84.9Å c: 95.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.268
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Molecular determinants for the stereospecific reduction of 3-ketosteroids and reactivity towards all-trans-retinal of a short-chain dehydrogenase/reductase (DHRS4).
Endo et al. (2009)
1 more

1 mention without citation

Structure of 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21) holoenzyme from an orthorhombic crystal form: an insight into the bifunctionality of the enzyme.
Dhagat et al. (2007)