› 2p4t

X-ray diffraction
1.15Å resolution

Structure of the Q67H mutant of R67 dihydrofolate reductase-NADP+ complex reveals a novel cofactor binding mode

Released:
DOI: 10.2210/pdb2p4t/pdb
PDB 2p4t coloured by chain and viewed from the front.
PDB 2p4t coloured by chain and viewed from the side.
PDB 2p4t coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structure of the Q67H mutant of R67 dihydrofolate reductase-NADP+ complex reveals a novel cofactor binding mode.
Divya N, Grifith E, Narayana N
Protein Sci. 16 1063-8 (2007)
PMID: 17473013

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase type 2 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 6.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00383 (Residues: 17-78; Coverage: 80%)
Sequence domains: R67 dihydrofolate reductase
Structure domains: SH3 type barrels.

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: I4122
Unit cell:
a: 67.46Å b: 67.46Å c: 52.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.19 0.215
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Crystal structure of a type II dihydrofolate reductase catalytic ternary complex.
Krahn et al. (2007)

1 mention without citation

Structure of the Q67H mutant of R67 dihydrofolate reductase-NADP+ complex reveals a novel cofactor binding mode.
Divya et al. (2007)