2p4a

X-ray diffraction
1.9Å resolution

X-ray structure of a camelid affinity matured single-domain vhh antibody fragment in complex with RNASE A

Released:

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-106850 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease pancreatic Chains: A, C
Molecule details ›
Chains: A, C
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain
Ig-like domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 121 amino acids
Theoretical weight: 12.83 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
UniProt:
  • Canonical: A2KD57 (Residues: 1-132; Coverage: 92%)
Gene name: hi113
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 70.027Å b: 131.623Å c: 137.156Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.218
Expression system: Escherichia coli