2p1o

X-ray diffraction
1.9Å resolution

Mechanism of Auxin Perception by the TIR1 ubiquitin ligase

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SKP1-like protein 1A Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 17.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q39255 (Residues: 1-160; Coverage: 100%)
Gene names: ASK1, At1g75950, SKP1, SKP1A, T4O12.17, UIP1
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
Protein TRANSPORT INHIBITOR RESPONSE 1 Chain: B
Molecule details ›
Chain: B
Length: 594 amino acids
Theoretical weight: 66.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q570C0 (Residues: 1-594; Coverage: 100%)
Gene names: At3g62980, FBL1, T20O10.80, TIR1, WEI1
Sequence domains: Transport inhibitor response 1 protein domain
Structure domains:
Auxin-responsive protein IAA7 Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.6 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: Q38825 (Residues: 82-94; Coverage: 5%)
Gene names: AXR2, At3g23050, IAA7, MXC7.8

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 101.971Å b: 82.456Å c: 125.32Å
α: 90° β: 103.84° γ: 90°
R-values:
R R work R free
0.186 0.184 0.231
Expression systems:
  • Spodoptera frugiperda
  • Not provided