2p1n

X-ray diffraction
2.5Å resolution

Mechanism of Auxin Perception by the TIR1 Ubiqutin Ligase

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SKP1-like protein 1A Chains: A, D
Molecule details ›
Chains: A, D
Length: 160 amino acids
Theoretical weight: 17.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q39255 (Residues: 1-160; Coverage: 100%)
Gene names: ASK1, At1g75950, SKP1, SKP1A, T4O12.17, UIP1
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
Protein TRANSPORT INHIBITOR RESPONSE 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 594 amino acids
Theoretical weight: 66.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q570C0 (Residues: 1-594; Coverage: 100%)
Gene names: At3g62980, FBL1, T20O10.80, TIR1, WEI1
Sequence domains: Transport inhibitor response 1 protein domain
Structure domains:
Auxin-responsive protein IAA7 Chains: C, F
Molecule details ›
Chains: C, F
Length: 13 amino acids
Theoretical weight: 1.6 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: Q38825 (Residues: 82-94; Coverage: 5%)
Gene names: AXR2, At3g23050, IAA7, MXC7.8

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 102.665Å b: 82.745Å c: 125.791Å
α: 90° β: 100.4° γ: 90°
R-values:
R R work R free
0.199 0.195 0.274
Expression systems:
  • Spodoptera frugiperda
  • Not provided