2ozm

X-ray diffraction
2.86Å resolution

Crystal structure of RB69 gp43 in complex with DNA with 5-NITP opposite an abasic site analog

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 903 amino acids
Theoretical weight: 104.66 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q38087 (Residues: 1-903; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
Template DNA Chain: T
Molecule details ›
Chain: T
Length: 18 nucleotides
Theoretical weight: 5.37 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided
Primer DNA Chain: P
Molecule details ›
Chain: P
Length: 14 nucleotides
Theoretical weight: 4.3 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 81.232Å b: 117.492Å c: 126.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.204 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided