2oxc

X-ray diffraction
1.3Å resolution

Human DEAD-box RNA helicase DDX20, DEAD domain in complex with ADP

Released:
Source organism: Homo sapiens
Entry authors: Karlberg T, Ogg D, Arrowsmith CH, Berglund H, Busam RD, Collins R, Dahlgren LG, Edwards A, Flodin S, Flores A, Graslund S, Hallberg BM, Hammarstrom M, Hogbom M, Johansson I, Kotenyova T, Lehtio L, Moche M, Nordlund P, Nyman T, Persson C, Sagemark J, Stenmark P, Sundstrom M, Thorsell AG, Van Den Berg S, Weigelt J, Holmberg-Schiavone L, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable ATP-dependent RNA helicase DDX20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 25.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHI6 (Residues: 41-268; Coverage: 28%)
Gene names: DDX20, DP103, GEMIN3
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3121
Unit cell:
a: 63.8Å b: 63.8Å c: 214.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.153 0.175
Expression system: Escherichia coli