› 2ovb

X-ray diffraction
2.61Å resolution

Crystal Structure of StaL-sulfate complex

Released:
DOI: 10.2210/pdb2ovb/pdb
PDB entry 2ovb coloured by chain, front view.
PDB entry 2ovb coloured by chain, side view.
PDB entry 2ovb coloured by chain, top view.
Source organism: Streptomyces toyocaensis
Primary publication:
Crystal structure of StaL, a glycopeptide antibiotic sulfotransferase from Streptomyces toyocaensis.
Shi R, Lamb SS, Bhat S, Sulea T, Wright GD, Matte A, Cygler M
J Biol Chem 282 13073-86 (2007)
PMID: 17329243

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + desulfo-A47934 = adenosine 3',5'-bisphosphate + A47934
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Desulfo-A47934 sulfotransferase Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 32.21 KDa
Source organism: Streptomyces toyocaensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KLM3 (Residues: 4-270; Coverage: 99%)
Gene names: BU52_01305, staL
Sequence domains: Sulfotransferase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, null
Spacegroup: P6122
Unit cell:
a: 88.733Å b: 88.733Å c: 169.691Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.269
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Sulfotransferases, sulfatases and formylglycine-generating enzymes: a sulfation fascination.
Bojarová et al. (2008)
3 more