› 2otx

X-ray diffraction
2.6Å resolution

Crystal Structure of A N-terminal Fragment of SKAP-HOM Containing both the Helical Dimerization Domain and the PH Domain

Released:
DOI: 10.2210/pdb2otx/pdb
PDB entry 2otx coloured by chain, front view.
PDB entry 2otx coloured by chain, side view.
PDB entry 2otx coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The Skap-hom dimerization and PH domains comprise a 3'-phosphoinositide-gated molecular switch.
Swanson KD, Tang Y, Ceccarelli DF, Poy F, Sliwa JP, Neel BG, Eck MJ
Mol Cell 32 564-75 (2008)
PMID: 19026786

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Src kinase-associated phosphoprotein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 24.17 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3UND0 (Residues: 12-222; Coverage: 59%)
Gene names: Prap, Ra70, Saps, Scap2, Skap2, Skap55r
Sequence domains: PH domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 106.205Å b: 56.742Å c: 89.115Å
α: 90° β: 95.82° γ: 90°
R-values:
R R work R free
0.181 0.179 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Pleckstrin homology (PH) like domains - versatile modules in protein-protein interaction platforms.
Scheffzek et al. (2012)
8 more

5 mentions without citation

The Skap-hom dimerization and PH domains comprise a 3'-phosphoinositide-gated molecular switch.
Swanson et al. (2008)
4 more