› 2otw

X-ray diffraction
2.35Å resolution

Crystal structure of Fv polyglutamine complex

Released:
DOI: 10.2210/pdb2otw/pdb
PDB entry 2otw coloured by chain, front view.
PDB entry 2otw coloured by chain, side view.
PDB entry 2otw coloured by chain, top view.
Source organism: Mus musculus
Entry author: Li P

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211599 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fv light chain avriable domain VL Chains: A, C
Molecule details ›
Chains: A, C
Length: 115 amino acids
Theoretical weight: 12.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Fv heavy chain variable domain VH Chains: B, D
Molecule details ›
Chains: B, D
Length: 118 amino acids
Theoretical weight: 13.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
poly-Gln peptide antigen Chains: E, F
Molecule details ›
Chains: E, F
Length: 12 amino acids
Theoretical weight: 1.41 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 165.29Å b: 78.724Å c: 49.997Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.258
Expression system: Escherichia coli BL21(DE3)

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