› 2or2

X-ray diffraction
1.84Å resolution

Structure of the W47A/W242A Mutant of Bacterial Phosphatidylinositol-Specific Phospholipase C

Released:
DOI: 10.2210/pdb2or2/pdb
PDB entry 2or2 coloured by chain, front view.
PDB entry 2or2 coloured by chain, side view.
PDB entry 2or2 coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C.
Shao C, Shi X, Wehbi H, Zambonelli C, Head JF, Seaton BA, Roberts MF
J Biol Chem 282 9228-35 (2007)
PMID: 17213187

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 34.06 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08954 (Residues: 32-327; Coverage: 99%)
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 64.424Å b: 70.028Å c: 154.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.215
Expression system: Escherichia coli BL21(DE3)

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Citations

7 citation in other articles

Dual role of the second extracellular loop of the cannabinoid receptor 1: ligand binding and receptor localization.
Ahn et al. (2009)
6 more

7 mentions without citation

Towards a structural understanding of allosteric drugs at the human calcium-sensing receptor.
Leach et al. (2016)
6 more