› 2oqw

X-ray diffraction
2.1Å resolution

The crystal structure of sortase B from B.anthracis in complex with AAEK1

Released:
DOI: 10.2210/pdb2oqw/pdb
PDB 2oqw coloured by chain and viewed from the front.
PDB 2oqw coloured by chain and viewed from the side.
PDB 2oqw coloured by chain and viewed from the top.
Source organism: Bacillus anthracis str. Ames
Primary publication:
Activation of inhibitors by sortase triggers irreversible modification of the active site.
Maresso AW, Wu R, Kern JW, Zhang R, Janik D, Missiakas DM, Duban ME, Joachimiak A, Schneewind O
J. Biol. Chem. 282 23129-39 (2007)
PMID: 17545669

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SrtB family sortase Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 26.93 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L8PZR0 (Residues: 35-254; Coverage: 87%)
Gene name: GBAA_4783
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 10 x MSE
Ligand CS3 1 x CS3

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 34.436Å b: 73.806Å c: 89.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.259
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Targeting Bacterial Sortases in Search of Anti-Virulence Therapies with Low Risk of Resistance Development.
Nitulescu et al. (2021)
14 more

4 mentions without citation

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
3 more