X-ray diffraction
1.9Å resolution

Tandem SH2 domains of ZAP-70 with 19-mer zeta1 peptide


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ZAP-70 Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P43403 (Residues: 3-256; Coverage: 41%)
Gene names: SRK, ZAP70
Sequence domains: SH2 domain
Structure domains:
T-cell surface glycoprotein CD3 zeta chain Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P20963 (Residues: 69-87; Coverage: 13%)
Gene names: CD247, CD3Z, T3Z, TCRZ

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 50.11Å b: 63.37Å c: 54Å
α: 90° β: 114.44° γ: 90°
R R work R free
0.209 0.209 0.255
Expression systems:
  • Escherichia coli
  • Not provided