2opa

X-ray diffraction
2.4Å resolution

YwhB binary complex with 2-Fluoro-p-hydroxycinnamate

Released:
Source organism: Bacillus subtilis
Entry authors: Hackert ML, Whitman CP, Almrud JJ

Function and Biology Details

Reaction catalysed:
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-hydroxymuconate tautomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 61 amino acids
Theoretical weight: 7.03 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P70994 (Residues: 2-62; Coverage: 98%)
Gene names: BSU37540, ywhB
Sequence domains: Tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I432
Unit cell:
a: 141.339Å b: 141.339Å c: 141.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.249
Expression system: Escherichia coli