› 2oox

X-ray diffraction
2.6Å resolution

Crystal structure of the adenylate sensor from AMP-activated protein kinase complexed with AMP

Released:
DOI: 10.2210/pdb2oox/pdb
PDB 2oox coloured by chain and viewed from the front.
PDB 2oox coloured by chain and viewed from the side.
PDB 2oox coloured by chain and viewed from the top.
Source organism: Schizosaccharomyces pombe
Entry authors: Townley R, Shapiro L

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SNF1-like protein kinase ssp2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 137 amino acids
Theoretical weight: 15.9 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O74536 (Residues: 440-576; Coverage: 24%)
Gene names: SPCC74.03c, ssp2
Sequence domains: Adenylate sensor of SNF1-like protein kinase
Structure domains: Kinase associated domain 1, KA1
5'-AMP-activated protein kinase subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 97 amino acids
Theoretical weight: 10.87 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P78789 (Residues: 203-298; Coverage: 32%)
Gene names: SPCC1919.03c, amk2
Sequence domains: 5'-AMP-activated protein kinase beta subunit, interaction domain
Structure domains: N-terminal domain of TfIIb
5'-AMP-activated protein kinase subunit gamma Chains: E, G
Molecule details ›
Chains: E, G
Length: 333 amino acids
Theoretical weight: 37.36 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q10343 (Residues: 2-334; Coverage: 100%)
Gene names: SPAC1556.08c, SPAC1F12.01c, cbs2
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments

1 bound ligand:
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 73.456Å b: 97.392Å c: 168.884Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.206 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

AMPK-Mediated Regulation of Lipid Metabolism by Phosphorylation.
Wang et al. (2018)
34 more

5 mentions without citation

RASMOT-3D PRO: a 3D motif search webserver.
Debret et al. (2009)
4 more