2oox

X-ray diffraction
2.6Å resolution

Crystal structure of the adenylate sensor from AMP-activated protein kinase complexed with AMP

Released:
Source organism: Schizosaccharomyces pombe
Entry authors: Townley R, Shapiro L

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SNF1-like protein kinase ssp2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 137 amino acids
Theoretical weight: 15.9 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O74536 (Residues: 440-576; Coverage: 24%)
Gene names: SPCC74.03c, ssp2
Sequence domains: Adenylate sensor of SNF1-like protein kinase
Structure domains: Kinase associated domain 1, KA1
5'-AMP-activated protein kinase subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 97 amino acids
Theoretical weight: 10.87 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P78789 (Residues: 203-298; Coverage: 32%)
Gene names: SPCC1919.03c, amk2
Sequence domains: 5'-AMP-activated protein kinase beta subunit, interaction domain
Structure domains: N-terminal domain of TfIIb
5'-AMP-activated protein kinase subunit gamma Chains: E, G
Molecule details ›
Chains: E, G
Length: 333 amino acids
Theoretical weight: 37.36 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q10343 (Residues: 2-334; Coverage: 100%)
Gene names: SPAC1556.08c, SPAC1F12.01c, cbs2
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 73.456Å b: 97.392Å c: 168.884Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.206 0.273
Expression system: Escherichia coli BL21(DE3)