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ABC transporter ModBC in complex with its binding protein ModA

X-ray diffraction
3.1Å resolution
PDB entry 2onk coloured by chain, front view.
PDB entry 2onk coloured by chain, side view.
PDB entry 2onk coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Assembly composition:
hetero pentamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 52071.93 Å2
Buried surface area: 17636.05 Å2
Dissociation area: 1,485.53 Å2
Dissociation energy (ΔGdiss): 1 kcal/mol
Dissociation entropy (TΔSdiss): 13.85 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-128224
Assembly 2
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Multimeric state: hetero pentamer
Accessible surface area: 52671.59 Å2
Buried surface area: 17326.11 Å2
Dissociation area: 1,340.92 Å2
Dissociation energy (ΔGdiss): -1.38 kcal/mol
Dissociation entropy (TΔSdiss): 13.83 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-128224

Macromolecules

Molybdate/tungstate import ATP-binding protein WtpC
Chains: A, B, F, G
Length: 240 amino acids
Theoretical weight: 27.07 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30144 (Residues: 1-240; Coverage: 100%)
Gene names: AF_0092, modC, wtpC
Pfam: ABC transporter
InterPro:
CATH: P-loop containing nucleotide triphosphate hydrolases
SCOP: ABC transporter ATPase domain-like
Molybdate/tungstate import ATP-binding protein WtpC in PDB entry 2onk, assembly 1, front view.
Molybdate/tungstate import ATP-binding protein WtpC in PDB entry 2onk, assembly 1, side view.
Molybdate/tungstate import ATP-binding protein WtpC in PDB entry 2onk, assembly 1, top view.
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Molybdate/tungstate transport system permease protein WtpB
Chains: C, D, H, I
Length: 284 amino acids
Theoretical weight: 30.64 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30143 (Residues: 1-261; Coverage: 100%)
Gene names: AF_0093, modB, wtpB
Pfam: Binding-protein-dependent transport system inner membrane component
InterPro:
CATH: MetI-like
SCOP: MetI-like
Molybdate/tungstate transport system permease protein WtpB in PDB entry 2onk, assembly 1, front view.
Molybdate/tungstate transport system permease protein WtpB in PDB entry 2onk, assembly 1, side view.
Molybdate/tungstate transport system permease protein WtpB in PDB entry 2onk, assembly 1, top view.
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Molybdate/tungstate-binding protein WtpA
Chains: E, J
Length: 314 amino acids
Theoretical weight: 35.72 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30142 (Residues: 31-342; Coverage: 98%)
Gene names: AF_0094, modA, wtpA
Pfam: Bacterial extracellular solute-binding protein
InterPro: Tungstate ABC transporter, substrate-binding protein WtpA
CATH: Periplasmic binding protein-like II
SCOP: Phosphate binding protein-like
Molybdate/tungstate-binding protein WtpA in PDB entry 2onk, assembly 1, front view.
Molybdate/tungstate-binding protein WtpA in PDB entry 2onk, assembly 1, side view.
Molybdate/tungstate-binding protein WtpA in PDB entry 2onk, assembly 1, top view.
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