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X-ray diffraction
3.1Å resolution

ABC transporter ModBC in complex with its binding protein ModA

Released:
DOI: 10.2210/pdb2onk/pdb
PDB entry 2onk coloured by chain, front view.
PDB entry 2onk coloured by chain, side view.
PDB entry 2onk coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structure of an ABC transporter in complex with its binding protein.
Hollenstein K, Frei DC, Locher KP
Nature 446 213-6 (2007)
PMID: 17322901

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + tungstate-[tungstate-binding protein](Side 1) = ADP + phosphate + tungstate(Side 2) + [tungstate-binding protein](Side 1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-128224 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Molybdate/tungstate import ATP-binding protein WtpC Chains: A, B, F, G
Molecule details ›
Chains: A, B, F, G
Length: 240 amino acids
Theoretical weight: 27.07 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30144 (Residues: 1-240; Coverage: 100%)
Gene names: AF_0092, modC, wtpC
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Molybdate/tungstate transport system permease protein WtpB Chains: C, D, H, I
Molecule details ›
Chains: C, D, H, I
Length: 284 amino acids
Theoretical weight: 30.64 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30143 (Residues: 1-261; Coverage: 100%)
Gene names: AF_0093, modB, wtpB
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
Molybdate/tungstate-binding protein WtpA Chains: E, J
Molecule details ›
Chains: E, J
Length: 314 amino acids
Theoretical weight: 35.72 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30142 (Residues: 31-342; Coverage: 98%)
Gene names: AF_0094, modA, wtpA
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:
Ligand PO4 4 x PO4
Ligand MG 6 x MG
Ligand WO4 2 x WO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 93.032Å b: 171.203Å c: 158.47Å
α: 90° β: 98.79° γ: 90°
R-values:
R R work R free
0.256 0.256 0.283
Expression system: Escherichia coli

Quick links

Citations

73 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
72 more

30 mentions without citation

ABC transporters: the power to change.
Rees et al. (2009)
29 more