2om7

Electron Microscopy
7.3Å resolution

Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-bound Elongation Factors

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-216005 (preferred)
Entry contents:
4 distinct polypeptide molecules
10 distinct RNA molecules
Macromolecules (14 distinct):
Small ribosomal subunit protein uS12 Chain: E
Molecule details ›
Chain: E
Length: 135 amino acids
Theoretical weight: 14.92 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHN3 (Residues: 2-132; Coverage: 99%)
Gene names: TTHA1697, rpsL
Sequence domains: Ribosomal protein S12/S23
Large ribosomal subunit protein uL1 Chain: K
Molecule details ›
Chain: K
Length: 229 amino acids
Theoretical weight: 24.87 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SLP7 (Residues: 1-229; Coverage: 100%)
Gene names: TTHA0246, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Elongation factor G Chain: L
Molecule details ›
Chain: L
Length: 691 amino acids
Theoretical weight: 76.98 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SHN5 (Residues: 1-691; Coverage: 100%)
Gene names: TTHA1695, fus, fusA
Sequence domains:
Small ribosomal subunit protein uS2 Chain: N
Molecule details ›
Chain: N
Length: 256 amino acids
Theoretical weight: 29.32 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80371 (Residues: 1-256; Coverage: 100%)
Gene names: TTHA0861, rps2, rpsB
Sequence domains: Ribosomal protein S2
Fragment of 16S rRNA (h14) Chain: A
Molecule details ›
Chain: A
Length: 12 nucleotides
Theoretical weight: 3.83 KDa
Fragment of 16S rRNA (h15) Chain: B
Molecule details ›
Chain: B
Length: 28 nucleotides
Theoretical weight: 9.05 KDa
Fragment of 16S rRNA (h44) Chain: C
Molecule details ›
Chain: C
Length: 96 nucleotides
Theoretical weight: 31.21 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
16S ribosomal RNA (H5) Chain: D
Molecule details ›
Chain: D
Length: 303 nucleotides
Theoretical weight: 98.29 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
Fragment of23S rRNA (H95) Chain: F
Molecule details ›
Chain: F
Length: 29 nucleotides
Theoretical weight: 9.38 KDa
Fragment of23S rRNA (H68) Chain: G
Molecule details ›
Chain: G
Length: 54 nucleotides
Theoretical weight: 17.63 KDa
Fragment of23S rRNA (H89) Chain: H
Molecule details ›
Chain: H
Length: 42 nucleotides
Theoretical weight: 13.54 KDa
Fragment of23S rRNA (H42-44) Chain: I
Molecule details ›
Chain: I
Length: 58 nucleotides
Theoretical weight: 18.71 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
Fragment of23S rRNA (H76) Chain: J
Molecule details ›
Chain: J
Length: 102 nucleotides
Theoretical weight: 33.15 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
p/E-tRNA Chain: M
Molecule details ›
Chain: M
Length: 74 nucleotides
Theoretical weight: 23.73 KDa
Sequence domains: tRNA

Ligands and Environments

No bound ligands

Experiments and Validation Details

Entry percentile scores
Resolution: 7.3Å
Relevant EMDB volumes: EMD-1315
Expression system: Escherichia coli BL21(DE3)