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X-ray diffraction
2.02Å resolution

Crystal Structure of the GEOBACILLUS STEAROTHERMOPHILUS Carboxylesterase EST55 at pH 6.2

Released:
DOI: 10.2210/pdb2ogs/pdb
PDB entry 2ogs coloured by chain, front view.
PDB entry 2ogs coloured by chain, side view.
PDB entry 2ogs coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure of the Geobacillus stearothermophilus carboxylesterase Est55 and its activation of prodrug CPT-11.
Liu P, Ewis HE, Tai PC, Lu CD, Weber IT
J Mol Biol 367 212-23 (2007)
PMID: 17239398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylic ester hydrolase Chain: A
Molecule details ›
Chain: A
Length: 498 amino acids
Theoretical weight: 54.97 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GCC7 (Residues: 1-498; Coverage: 100%)
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand IOD 3 x IOD
1 modified residue:
Ligand 0CS 1 x 0CS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 69.53Å b: 73.49Å c: 99.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.236
Expression system: Escherichia coli

Quick links

Citations

14 citation in other articles

Hydrolysis of cyclic poly(ethylene terephthalate) trimers by a carboxylesterase from Thermobifida fusca KW3.
Billig et al. (2010)
13 more

4 mentions without citation

Trimethyllysine: From Carnitine Biosynthesis to Epigenetics.
Maas et al. (2020)
3 more