2ody

X-ray diffraction
2.35Å resolution

Thrombin-bound boophilin displays a functional and accessible reactive-site loop

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Thrombin light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 49 amino acids
Theoretical weight: 5.74 KDa
Source organism: Bos taurus
UniProt:
  • Best match: P00735 (Residues: 318-366)
Gene name: F2
Thrombin heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 259 amino acids
Theoretical weight: 29.77 KDa
Source organism: Bos taurus
UniProt:
  • Best match: P00735 (Residues: 367-625)
Gene name: F2
Boophilin-H2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 127 amino acids
Theoretical weight: 14 KDa
Source organism: Rhipicephalus microplus
Expression system: Escherichia coli
UniProt:
  • Best match: Q8WPI2 (Residues: 16-142)
Gene name: H2

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 92.5Å b: 104.2Å c: 129.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.23
Expression system: Escherichia coli