› 2odl

X-ray diffraction
1.92Å resolution

Crystal structure of the HMW1 secretion domain from Haemophilus influenzae

Released:
DOI: 10.2210/pdb2odl/pdb
PDB entry 2odl coloured by chain, front view.
PDB entry 2odl coloured by chain, side view.
PDB entry 2odl coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
The structure of the Haemophilus influenzae HMW1 pro-piece reveals a structural domain essential for bacterial two-partner secretion.
Yeo HJ, Yokoyama T, Walkiewicz K, Kim Y, Grass S, Geme JW
J Biol Chem 282 31076-84 (2007)
PMID: 17699157

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Filamentous haemagglutinin FhaB/tRNA nuclease CdiA-like TPS domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 39.36 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q48031 (Residues: 69-441; Coverage: 24%)
Gene name: hmw1A
Sequence domains: TPS secretion domain
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I4
Unit cell:
a: 121.438Å b: 121.438Å c: 50.582Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.217
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Type V secretion: mechanism(s) of autotransport through the bacterial outer membrane.
Leo et al. (2012)
7 more

6 mentions without citation

Two-Partner Secretion: Combining Efficiency and Simplicity in the Secretion of Large Proteins for Bacteria-Host and Bacteria-Bacteria Interactions.
Guérin et al. (2017)
5 more