› 2ode

X-ray diffraction
1.9Å resolution

Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3

Released:
DOI: 10.2210/pdb2ode/pdb
PDB entry 2ode coloured by chain, front view.
PDB entry 2ode coloured by chain, side view.
PDB entry 2ode coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural diversity in the RGS domain and its interaction with heterotrimeric G protein alpha-subunits.
Soundararajan M, Willard FS, Kimple AJ, Turnbull AP, Ball LJ, Schoch GA, Gileadi C, Fedorov OY, Dowler EF, Higman VA, Hutsell SQ, Sundström M, Doyle DA, Siderovski DP
Proc Natl Acad Sci U S A 105 6457-62 (2008)
PMID: 18434541

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140369 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 350 amino acids
Theoretical weight: 40.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08754 (Residues: 4-350; Coverage: 98%)
Gene name: GNAI3
Sequence domains: G-protein alpha subunit
Structure domains:
Regulator of G-protein signaling 8 Chains: B, D
Molecule details ›
Chains: B, D
Length: 141 amino acids
Theoretical weight: 16.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P57771 (Residues: 42-180; Coverage: 77%)
Gene name: RGS8
Sequence domains: Regulator of G protein signaling domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ALF 2 x ALF
Ligand MG 2 x MG
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 112.837Å b: 130.216Å c: 68.519Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.211
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Update on melatonin receptors: IUPHAR Review 20.
Jockers et al. (2016)
21 more

23 mentions without citation

Regulators of G-protein signaling and their Gα substrates: promises and challenges in their use as drug discovery targets.
Kimple et al. (2011)
22 more