2ocz

X-ray diffraction
1.85Å resolution

The Structure of a Putative 3-Dehydroquinate Dehydratase from Streptococcus pyogenes.

Released:
Entry authors: Cuff ME, Duggan E, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
3-dehydroquinate = 3-dehydroshikimate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-dehydroquinate dehydratase Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 26.87 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63590 (Residues: 1-228; Coverage: 100%)
Gene names: M5005_Spy0624, SPy_0809, aroD
Sequence domains: Type I 3-dehydroquinase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 81.688Å b: 81.688Å c: 85.364Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.23
Expression system: Escherichia coli BL21(DE3)