› 2o4v

X-ray diffraction
1.94Å resolution

An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane

Released:
DOI: 10.2210/pdb2o4v/pdb
PDB entry 2o4v coloured by chain, front view.
PDB entry 2o4v coloured by chain, side view.
PDB entry 2o4v coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
An arginine ladder in OprP mediates phosphate-specific transfer across the outer membrane.
Moraes TF, Bains M, Hancock RE, Strynadka NC
Nat Struct Mol Biol 14 85-7 (2007)
PMID: 17187075

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138744 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin P Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 411 amino acids
Theoretical weight: 45.28 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: P05695 (Residues: 30-440; Coverage: 100%)
Gene names: PA3279, oprP
Sequence domains: Phosphate-selective porin O and P
Structure domains: Porin

Ligands and Environments

5 bound ligands:
Ligand CA 3 x CA
Ligand PO4 3 x PO4
Ligand CL 1 x CL
Ligand C8E 4 x C8E
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 255.13Å b: 142.769Å c: 62.025Å
α: 90° β: 99.49° γ: 90°
R-values:
R R work R free
0.175 0.174 0.194

Quick links

Citations

6 review citations

Microbe-host interactions: structure and role of Gram-negative bacterial porins.
Galdiero et al. (2012)
5 more

14 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
13 more