› 2o4c

X-ray diffraction
2.3Å resolution

Crystal Structure of D-Erythronate-4-phosphate Dehydrogenase Complexed with NAD

Released:
DOI: 10.2210/pdb2o4c/pdb
PDB entry 2o4c coloured by chain, front view.
PDB entry 2o4c coloured by chain, side view.
PDB entry 2o4c coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of D-erythronate-4-phosphate dehydrogenase complexed with NAD.
Ha JY, Lee JH, Kim KH, Kim DJ, Lee HH, Kim HK, Yoon HJ, Suh SW
J Mol Biol 366 1294-304 (2007)
PMID: 17217963

Function and Biology Details

Reaction catalysed: 
4-phospho-D-erythronate + NAD(+) = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Erythronate-4-phosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 380 amino acids
Theoretical weight: 41.05 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I3W9 (Residues: 1-380; Coverage: 100%)
Gene names: PA1375, pdxB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand PO4 1 x PO4
Ligand TLA 1 x TLA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 84.914Å b: 101.63Å c: 142.994Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.225 0.259
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Coenzyme biosynthesis: enzyme mechanism, structure and inhibition.
Scott et al. (2007)
9 other articles

2 mentions without citation

Classification, substrate specificity and structural features of D-2-hydroxyacid dehydrogenases: 2HADH knowledgebase.
Matelska et al. (2018)
1 more