2o3b

X-ray diffraction
2.3Å resolution

Crystal structure complex of Nuclease A (NucA) with intra-cellular inhibitor NuiA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclease Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 26.38 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: P38446 (Residues: 35-274; Coverage: 96%)
Gene names: all7362, nucA
Sequence domains: DNA/RNA non-specific endonuclease
Structure domains: Extracellular Endonuclease, subunit A
Sugar-non-specific nuclease inhibitor Chain: B
Molecule details ›
Chain: B
Length: 136 amino acids
Theoretical weight: 15.13 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7A260 (Residues: 2-135; Coverage: 99%)
Gene name: nuiA
Sequence domains: Nuclease A inhibitor-like protein
Structure domains: Nuclease A inhibitor-like

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 87.225Å b: 87.225Å c: 138.977Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.241
Expression system: Escherichia coli