› 2o1e

X-ray diffraction
2.6Å resolution

Crystal Structure of the Metal-dependent Lipoprotein YcdH from Bacillus subtilis, Northeast Structural Genomics Target SR583

Released:
DOI: 10.2210/pdb2o1e/pdb
PDB 2o1e coloured by chain and viewed from the front.
PDB 2o1e coloured by chain and viewed from the side.
PDB 2o1e coloured by chain and viewed from the top.
Source organism: Bacillus subtilis
Entry authors: Forouhar F, Zhou W, Seetharaman J, Chen CX, Fang Y, Cunningham K, Ma L-C, Janjua H, Xiao R, Baran MC, Liu J, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
High-affinity zinc uptake system binding-protein ZnuA Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.46 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34966 (Residues: 22-319; Coverage: 100%)
Gene names: BSU02850, adcA, ycdH, znuA
Sequence domains: Zinc-uptake complex component A periplasmic
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P1
Unit cell:
a: 45.292Å b: 54.344Å c: 62.84Å
α: 103.7° β: 100.46° γ: 96.75°
R-values:
R R work R free
0.223 0.223 0.29
Expression system: Escherichia coli

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