› 2o08

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A PUTATIVE HD SUPERFAMILY HYDROLASE (BH1327) FROM BACILLUS HALODURANS AT 1.90 A RESOLUTION

Released:
DOI: 10.2210/pdb2o08/pdb
PDB entry 2o08 coloured by chain, front view.
PDB entry 2o08 coloured by chain, side view.
PDB entry 2o08 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
P(1),P(4)-bis(5'-adenosyl) tetraphosphate + H(2)O = 2 ADP
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191843 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bis(5'-nucleosyl)-tetraphosphatase, symmetrical Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.79 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KD90 (Residues: 1-187; Coverage: 100%)
Gene name: BH1327
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

6 bound ligands:
Ligand FE 4 x FE
Ligand PO4 1 x PO4
Ligand UNL 1 x UNL
Ligand PG4 7 x PG4
Ligand NO3 1 x NO3
Ligand DGI 1 x DGI
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5, APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 86.83Å b: 166.51Å c: 73.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.223
Expression system: Escherichia coli

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