› 2nz2

X-ray diffraction
2.4Å resolution

Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline

Released:
DOI: 10.2210/pdb2nz2/pdb
PDB 2nz2 coloured by chain and viewed from the front.
PDB 2nz2 coloured by chain and viewed from the side.
PDB 2nz2 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structure of human argininosuccinate synthetase.
Karlberg T, Collins R, van den Berg S, Flores A, Hammarstr��m M, H��gbom M, Holmberg Schiavone L, Uppenberg J
Acta Crystallogr. D Biol. Crystallogr. 64 279-86 (2008)
PMID: 18323623

Function and Biology Details

Reaction catalysed: 
ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate synthase Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 46.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00966 (Residues: 1-412; Coverage: 100%)
Gene names: ASS, ASS1
Sequence domains: Arginosuccinate synthase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand ASP 1 x ASP
Ligand CIR 1 x CIR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: F222
Unit cell:
a: 95.94Å b: 117.47Å c: 155.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.265
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

A structural perspective on the PP-loop ATP pyrophosphatase family.
Fellner et al. (2018)
5 more

6 mentions without citation

Plumbagin elicits differential proteomic responses mainly involving cell cycle, apoptosis, autophagy, and epithelial-to-mesenchymal transition pathways in human prostate cancer PC-3 and DU145 cells.
Qiu et al. (2015)
5 more