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Rop4-GDP-PRONE8

X-ray diffraction
3.1Å resolution
PDB entry 2nty coloured by chain, front view.
PDB entry 2nty coloured by chain, side view.
PDB entry 2nty coloured by chain, top view.
Source organism: Arabidopsis thaliana
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Rho guanine nucleotide exchange factor 8 and Rac-like GTP-binding protein ARAC5
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Multimeric state: hetero tetramer
Accessible surface area: 43776.34 Å2
Buried surface area: 12120.18 Å2
Dissociation area: 1,993.69 Å2
Dissociation energy (ΔGdiss): 21.86 kcal/mol
Dissociation entropy (TΔSdiss): 14.96 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-174379

Macromolecules

Rho guanine nucleotide exchange factor 8
Chains: A, B
Length: 365 amino acids
Theoretical weight: 41.52 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LV40 (Residues: 76-440; Coverage: 70%)
Gene names: At3g24620, MOB24.21, ROPGEF8
Pfam: PRONE (Plant-specific Rop nucleotide exchanger)
InterPro:
CATH:
Rho guanine nucleotide exchange factor 8 in PDB entry 2nty, assembly 1, front view.
Rho guanine nucleotide exchange factor 8 in PDB entry 2nty, assembly 1, side view.
Rho guanine nucleotide exchange factor 8 in PDB entry 2nty, assembly 1, top view.
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Rac-like GTP-binding protein ARAC5
Chains: C, D
Length: 180 amino acids
Theoretical weight: 19.83 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38937 (Residues: 1-180; Coverage: 92%)
Gene names: ARAC5, ATGP3, At1g75840, ROP4, T4O12.8
Pfam: Ras family
InterPro:
CATH: P-loop containing nucleotide triphosphate hydrolases
Rac-like GTP-binding protein ARAC5 in PDB entry 2nty, assembly 1, front view.
Rac-like GTP-binding protein ARAC5 in PDB entry 2nty, assembly 1, side view.
Rac-like GTP-binding protein ARAC5 in PDB entry 2nty, assembly 1, top view.
Molecule details ›

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