2ntc › Citations

The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA.

Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A
PLoS Biol 5 e23 (2007)
Cited: 43 times
EuropePMC logo PMID: 17253903

Abstract

DNA replication is initiated upon binding of "initiators" to origins of replication. In simian virus 40 (SV40), the core origin contains four pentanucleotide binding sites organized as pairs of inverted repeats. Here we describe the crystal structures of the origin binding domain (obd) of the SV40 large T-antigen (T-ag) both with and without a subfragment of origin-containing DNA. In the co-structure, two T-ag obds are oriented in a head-to-head fashion on the same face of the DNA, and each T-ag obd engages the major groove. Although the obds are very close to each other when bound to this DNA target, they do not contact one another. These data provide a high-resolution structural model that explains site-specific binding to the origin and suggests how these interactions help direct the oligomerization events that culminate in assembly of the helicase-active dodecameric complex of T-ag.

Reviews - 2ntc mentioned but not cited (1)

  1. The critical protein interactions and structures that elicit growth deregulation in cancer and viral replication. Ou HD, May AP, O'Shea CC. Wiley Interdiscip Rev Syst Biol Med 3 48-73 (2011)

Articles - 2ntc mentioned but not cited (10)

  1. The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA. Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A. PLoS Biol 5 e23 (2007)
  2. Asymmetric assembly of Merkel cell polyomavirus large T-antigen origin binding domains at the viral origin. Harrison CJ, Meinke G, Kwun HJ, Rogalin H, Phelan PJ, Bullock PA, Chang Y, Moore PS, Bohm A. J Mol Biol 409 529-542 (2011)
  3. Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158. Boer DR, Ruiz-Masó JA, Rueda M, Petoukhov MV, Machón C, Svergun DI, Orozco M, del Solar G, Coll M. Sci Rep 6 20915 (2016)
  4. Insights into the initiation of JC virus DNA replication derived from the crystal structure of the T-antigen origin binding domain. Meinke G, Phelan PJ, Kalekar R, Shin J, Archambault J, Bohm A, Bullock PA. PLoS Pathog 10 e1003966 (2014)
  5. Polyomavirus large T antigen binds symmetrical repeats at the viral origin in an asymmetrical manner. Harrison C, Jiang T, Banerjee P, Meinke G, D'Abramo CM, Schaffhausen B, Bohm A. J Virol 87 13751-13759 (2013)
  6. High performance transcription factor-DNA docking with GPU computing. Wu J, Hong B, Takeda T, Guo JT. Proteome Sci 10 Suppl 1 S17 (2012)
  7. Analysis of the costructure of the simian virus 40 T-antigen origin binding domain with site I reveals a correlation between GAGGC spacing and spiral assembly. Meinke G, Phelan PJ, Harrison CJ, Bullock PA. J Virol 87 2923-2934 (2013)
  8. Structural Based Analyses of the JC Virus T-Antigen F258L Mutant Provides Evidence for DNA Dependent Conformational Changes in the C-Termini of Polyomavirus Origin Binding Domains. Meinke G, Phelan PJ, Shin J, Gagnon D, Archambault J, Bohm A, Bullock PA. PLoS Pathog 12 e1005362 (2016)
  9. Structure-based design of a disulfide-linked oligomeric form of the simian virus 40 (SV40) large T antigen DNA-binding domain. Meinke G, Phelan P, Fradet-Turcotte A, Archambault J, Bullock PA. Acta Crystallogr D Biol Crystallogr 67 560-567 (2011)
  10. pyDockDNA: A new web server for energy-based protein-DNA docking and scoring. Rodríguez-Lumbreras LA, Jiménez-García B, Giménez-Santamarina S, Fernández-Recio J. Front Mol Biosci 9 988996 (2022)


Reviews citing this publication (11)

  1. The Mcm complex: unwinding the mechanism of a replicative helicase. Bochman ML, Schwacha A. Microbiol Mol Biol Rev 73 652-683 (2009)
  2. Merkel cell polyomavirus: a newly discovered human virus with oncogenic potential. Spurgeon ME, Lambert PF. Virology 435 118-130 (2013)
  3. Large T antigens of polyomaviruses: amazing molecular machines. An P, Sáenz Robles MT, Pipas JM. Annu Rev Microbiol 66 213-236 (2012)
  4. Replication and partitioning of papillomavirus genomes. McBride AA. Adv Virus Res 72 155-205 (2008)
  5. SV40 DNA replication: from the A gene to a nanomachine. Fanning E, Zhao K. Virology 384 352-359 (2009)
  6. AAA+ ATPases in the initiation of DNA replication. Duderstadt KE, Berger JM. Crit Rev Biochem Mol Biol 43 163-187 (2008)
  7. A structural framework for replication origin opening by AAA+ initiation factors. Duderstadt KE, Berger JM. Curr Opin Struct Biol 23 144-153 (2013)
  8. The large tumor antigen: a "Swiss Army knife" protein possessing the functions required for the polyomavirus life cycle. Topalis D, Andrei G, Snoeck R. Antiviral Res 97 122-136 (2013)
  9. Loading strategies of ring-shaped nucleic acid translocases and helicases. O'Shea VL, Berger JM. Curr Opin Struct Biol 25 16-24 (2014)
  10. Mechanisms of hexameric helicases. Fernandez AJ, Berger JM. Crit Rev Biochem Mol Biol 56 621-639 (2021)
  11. Structural Mechanisms of Hexameric Helicase Loading, Assembly, and Unwinding. Trakselis MA. F1000Res 5 F1000 Faculty Rev-111 (2016)

Articles citing this publication (21)

  1. Structure and conformational change of a replication protein A heterotrimer bound to ssDNA. Fan J, Pavletich NP. Genes Dev 26 2337-2347 (2012)
  2. High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. Jäger V, Büssow K, Wagner A, Weber S, Hust M, Frenzel A, Schirrmann T. BMC Biotechnol 13 52 (2013)
  3. The minimum replication origin of merkel cell polyomavirus has a unique large T-antigen loading architecture and requires small T-antigen expression for optimal replication. Kwun HJ, Guastafierro A, Shuda M, Meinke G, Bohm A, Moore PS, Chang Y. J Virol 83 12118-12128 (2009)
  4. Origin remodeling and opening in bacteria rely on distinct assembly states of the DnaA initiator. Duderstadt KE, Mott ML, Crisona NJ, Chuang K, Yang H, Berger JM. J Biol Chem 285 28229-28239 (2010)
  5. Mechanism of origin DNA recognition and assembly of an initiator-helicase complex by SV40 large tumor antigen. Chang YP, Xu M, Machado AC, Yu XJ, Rohs R, Chen XS. Cell Rep 3 1117-1127 (2013)
  6. Conformational rearrangements of SV40 large T antigen during early replication events. Cuesta I, Núñez-Ramírez R, Scheres SH, Gai D, Chen XS, Fanning E, Carazo JM. J Mol Biol 397 1276-1286 (2010)
  7. Model for T-antigen-dependent melting of the simian virus 40 core origin based on studies of the interaction of the beta-hairpin with DNA. Kumar A, Meinke G, Reese DK, Moine S, Phelan PJ, Fradet-Turcotte A, Archambault J, Bohm A, Bullock PA. J Virol 81 4808-4818 (2007)
  8. Quantitative analysis of the binding of simian virus 40 large T antigen to DNA. Fradet-Turcotte A, Vincent C, Joubert S, Bullock PA, Archambault J. J Virol 81 9162-9174 (2007)
  9. A specific docking site for DNA polymerase {alpha}-primase on the SV40 helicase is required for viral primosome activity, but helicase activity is dispensable. Huang H, Zhao K, Arnett DR, Fanning E. J Biol Chem 285 33475-33484 (2010)
  10. The structure of SV40 large T hexameric helicase in complex with AT-rich origin DNA. Gai D, Wang D, Li SX, Chen XS. Elife 5 e18129 (2016)
  11. Wild-type p53 enhances efficiency of simian virus 40 large-T-antigen-induced cellular transformation. Hermannstädter A, Ziegler C, Kühl M, Deppert W, Tolstonog GV. J Virol 83 10106-10118 (2009)
  12. SV40 T antigen interactions with ssDNA and replication protein A: a regulatory role of T antigen monomers in lagging strand DNA replication. Onwubiko NO, Borst A, Diaz SA, Passkowski K, Scheffel F, Tessmer I, Nasheuer HP. Nucleic Acids Res 48 3657-3677 (2020)
  13. Simian virus 40 DNA replication is dependent on an interaction between topoisomerase I and the C-terminal end of T antigen. Khopde S, Simmons DT. J Virol 82 1136-1145 (2008)
  14. Structure-based analysis of the interaction between the simian virus 40 T-antigen origin binding domain and single-stranded DNA. Meinke G, Phelan PJ, Fradet-Turcotte A, Bohm A, Archambault J, Bullock PA. J Virol 85 818-827 (2011)
  15. Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China. Liu P, Qiu Y, Xing C, Zhou JH, Yang WH, Wang Q, Li JY, Han X, Zhang YZ, Ge XY. Virol J 16 35 (2019)
  16. Analysis of JC virus DNA replication using a quantitative and high-throughput assay. Shin J, Phelan PJ, Chhum P, Bashkenova N, Yim S, Parker R, Gagnon D, Gjoerup O, Archambault J, Bullock PA. Virology 468-470 113-125 (2014)
  17. Evidence for a structural relationship between BRCT domains and the helicase domains of the replication initiators encoded by the Polyomaviridae and Papillomaviridae families of DNA tumor viruses. Kumar A, Joo WS, Meinke G, Moine S, Naumova EN, Bullock PA. J Virol 82 8849-8862 (2008)
  18. Analysis of DNA topology of EBV minichromosomes in HEK 293 cells. Castán A, Fernández-Calleja V, Hernández P, Krimer DB, Schvartzman JB, Fernández-Nestosa MJ. PLoS One 12 e0188172 (2017)
  19. Modeling of the SV40 DNA Replication Machine. Simmons DT. Genes (Basel) 3 742-758 (2012)
  20. TT(N)mGCCTC inhibits archaeal family B DNA polymerases. Sun S, Guo W, Yang JS, Qiu M, Zhu XJ, Dai ZM. Sci Rep 8 1990 (2018)
  21. Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity. Wan L, Toland S, Robinson-McCarthy LR, Lee N, Schaich MA, Hengel SR, Li X, Bernstein KA, Van Houten B, Chang Y, Moore PS. Proc Natl Acad Sci U S A 120 e2308010120 (2023)


Quick links