› 2nsj

X-ray diffraction
2.31Å resolution

E. coli PurE H45Q mutant complexed with CAIR

Released:
DOI: 10.2210/pdb2nsj/pdb
PDB entry 2nsj coloured by chain, front view.
PDB entry 2nsj coloured by chain, side view.
PDB entry 2nsj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
N5-CAIR mutase: role of a CO2 binding site and substrate movement in catalysis.
Hoskins AA, Morar M, Kappock TJ, Mathews II, Zaugg JB, Barder TE, Peng P, Okamoto A, Ealick SE, Stubbe J
Biochemistry 46 2842-55 (2007)
PMID: 17298082

Function and Biology Details

Reaction catalysed: 
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide mutase Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 17.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG18 (Residues: 1-169; Coverage: 100%)
Gene names: JW0512, b0523, purE
Sequence domains: AIR carboxylase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand C2R 1 x C2R
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I422
Unit cell:
a: 111.255Å b: 111.255Å c: 49.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.233
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
16 other articles

2 mentions without citation

Structure of complement C6 suggests a mechanism for initiation and unidirectional, sequential assembly of membrane attack complex (MAC).
Aleshin et al. (2012)
1 more