X-ray diffraction
2.55Å resolution

How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor


Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero pentamer
hetero tetramer (preferred)
hetero nonamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tetracycline repressor protein class B from transposon Tn10 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 23.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P04483 (Residues: 1-201; Coverage: 97%)
Gene name: tetR
Sequence domains:
Structure domains:
16 residue peptide Tip (Transcription inducing peptide) Chains: E, F, G, H, Z
Molecule details ›
Chains: E, F, G, H, Z
Length: 17 amino acids
Theoretical weight: 1.67 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21212
Unit cell:
a: 77.89Å b: 200.59Å c: 65.28Å
α: 90° β: 90° γ: 90°
R R work R free
0.211 0.206 0.27
Expression systems:
  • Escherichia coli
  • Not provided