2ns1

X-ray diffraction
1.96Å resolution

Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ammonium transporter AmtB Chain: A
Molecule details ›
Chain: A
Length: 412 amino acids
Theoretical weight: 42.95 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 23-428; Coverage: 100%)
Gene names: JW0441, amtB, b0451, ybaG
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains
Nitrogen regulatory protein GlnK Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 12.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC55 (Residues: 1-112; Coverage: 100%)
Gene names: JW0440, b0450, glnK, ybaI
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: R32
Unit cell:
a: 102.032Å b: 102.032Å c: 363.849Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.162 0.198
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)