2nps

X-ray diffraction
2.5Å resolution

Crystal Structure of the Early Endosomal SNARE Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Vesicle-associated membrane protein 4 Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.69 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O70480 (Residues: 47-117; Coverage: 50%)
Gene name: Vamp4
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-12 Chain: B
Molecule details ›
Chain: B
Length: 71 amino acids
Theoretical weight: 8.15 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3V7P1 (Residues: 184-251; Coverage: 25%)
Gene names: Stx12, Stx13
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Vesicle transport through interaction with t-SNAREs homolog 1A Chain: C
Molecule details ›
Chain: C
Length: 81 amino acids
Theoretical weight: 9.55 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JI51 (Residues: 122-199; Coverage: 35%)
Gene names: Vti1a, Vti1l2
Sequence domains: Snare region anchored in the vesicle membrane C-terminus
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-6 Chain: D
Molecule details ›
Chain: D
Length: 82 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43752 (Residues: 169-234; Coverage: 26%)
Gene name: STX6
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 252.882Å b: 28.657Å c: 41.887Å
α: 90° β: 98.25° γ: 90°
R-values:
R R work R free
0.251 0.247 0.289
Expression system: Escherichia coli BL21(DE3)